A novel lipase-based stationary phase in liquid chromatography

• Published in: Analytica chimica acta Vol. 689; no. 1; pp. 143 - 148
• Main Authors: He, Zeying, Lv, Chunguang, Fan, Xinxin, Zhou, Zhiqiang
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 09.03.2011; Elsevier
• Subjects: Alcohols - chemistry; Aminopropyl silica; Analytical chemistry; Asymmetrical hydrolysis; Asymmetry; Bioreactors; Candida antarctica; Candida antarctica lipase B; Catalysis; Chemistry; Chiral stationary phase; Chromatographic methods and physical methods associated with chromatography; Chromatography; Chromatography, High Pressure Liquid - instrumentation; Enzymes, Immobilized - chemistry; Esters; Exact sciences and technology; Fungal Proteins; Hydrolysis; Immobilized enzyme reactor; Lipase; Lipase - chemistry; Lipase - metabolism; Mathematical analysis; Other chromatographic methods; Silica gel; Silica Gel - chemistry; Silicon Dioxide - chemistry; Stereoisomerism; Triazoles - chemistry; Candida antarctica lipase B; Chiral stationary phase; Asymmetrical hydrolysis; Lipase; Immobilized enzyme reactor; Separation; Support; HPLC chromatography; Immobilization; Triacylglycerol lipase; Alcohol; Esterases; Liquid phase; Silica; Ester; Enantiomer; Silica gel; Stationary phase; Enzyme; Liquid chromatography; Method; Carboxylic ester hydrolases; Hydrolysis; Bioreactor; Hydrolases; Immobilized enzyme
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2011.01.025

In this paper, a new chiral stationary phase (CSP) based on Candida antarctica lipase B (CALB) bounding to the surface of macroporous silica gel was developed and its stereoselectivity in enantioseparation and asymmetrical hydrolysis was evaluated. Three CALB-based HPLC columns with different amounts of enzyme immobilized were prepared by employing the immobilization method, namely “in batch”. In this technique two chromatographic supports epoxy silica and aminopropyl silica were considered. This novel CSP was proven capable of hydrolyzing chiral esters asymmetrically as bioreactor and separating several aromatic alcohols and diniconazole enantiomers.