Activation of tyrosine aminotransferase expression in fetal liver by 5-azacytidine

Rat fetuses of 20 days gestational age were treated in utero with the inhibitor of DNA methylation, 5-azacytidine. The liver enzyme tyrosine aminotransferase, normally expressed at very low levels until several hours after birth, was increased by the drug in the fetal livers after a lag period of ab...

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Published inBiochemical and biophysical research communications Vol. 113; no. 2; pp. 645 - 649
Main Authors Rothrock, Robin, Perry, Stephanie T., Isham, Kenneth R., Lee, Kai-Lin, Kenney, Francis T.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.06.1983
Subjects
Animals
Azacitidine - pharmacology
Chemical Phenomena
Chemistry
Enzyme Activation - drug effects
Female
Fetus - enzymology
Liver - embryology
Liver - enzymology
Pregnancy
Rats
Rats, Inbred Strains
Tyrosine Transaminase - metabolism
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Summary:Rat fetuses of 20 days gestational age were treated in utero with the inhibitor of DNA methylation, 5-azacytidine. The liver enzyme tyrosine aminotransferase, normally expressed at very low levels until several hours after birth, was increased by the drug in the fetal livers after a lag period of about 9 hours, reaching a level 70-fold above control levels 18 hours after treatment. The high levels attained after 5-azacytidine treatment are comparable to those of glucocorticoid-treated adult livers, and were not further increased by administration of hydrocortisone to dams carrying treated fetuses. Cytidine and two other analogs, cytosine arabinoside and 6-azacytidine, were essentially without effect.
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(83)91775-8