Vibrio jasicida sp. nov., a member of the Harveyi clade, isolated from marine animals (packhorse lobster, abalone and Atlantic salmon)

• Published in: International journal of systematic and evolutionary microbiology Vol. 62; no. Pt 8; pp. 1864 - 1870
• Main Authors: Yoshizawa, Susumu, Tsuruya, Yasuhiro, Fukui, Youhei, Sawabe, Tomoo, Yokota, Akira, Kogure, Kazuhiro, Higgins, Melissa, Carson, Jeremy, Thompson, Fabiano L
• Format: Journal Article
• Language: English
• Published: Reading International Union of Microbiological Societies 01.08.2012; Society for General Microbiology
• Subjects: abalone; Animal, plant and microbial ecology; Animals; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; DNA; DNA, Bacterial - genetics; Fatty Acids - analysis; flagellum; Fundamental and applied biological sciences. Psychology; Gastropoda - microbiology; genes; Gram-negative bacteria; Haliotis; Jasus; lobsters; loci; Microbial ecology; Microbiology; Molecular Sequence Data; Multilocus Sequence Typing; new species; nucleic acid hybridization; nucleotide sequences; Palinuridae - microbiology; Phylogeny; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Salmo salar; Salmo salar - microbiology; Sequence Analysis, DNA; sodium chloride; Systematics; Various environments (extraatmospheric space, air, water); vertebrates; Vibrio - classification; Vibrio - genetics; Vibrio - isolation & purification; Vibrio alginolyticus; Vibrio campbellii; Vibrio natriegens; Vibrio parahaemolyticus; Abalone; Taxonomy; Salmo salar; Gastropoda; Macrura; Crustacea; Marine environment; Vertebrata; Lobster; Arthropoda; Pisces; Bacteria; Vibrionaceae; New species; Decapoda; Invertebrata; Mollusca; Vibrio
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.025916-0

Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (Jasus verreauxi), abalone (Haliotis sp.) and Atlantic salmon (Salmo salar), between 1994 and 2002. The bacteria were Gram-negative, rod-shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow in the presence of 0.5–8.0 % NaCl (optimum 3.0–6.0 %) and at 10–37 °C (optimum 25–30 °C). On the basis of 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA) using five loci (2443 bp; gyrB, pyrH, ftsZ, mreB and gapA), the closest phylogenetic neighbours of strain TCFB 0772T were the type strains of Vibrio communis (99.8 and 94.6 % similarity, respectively), Vibrio owensii (99.8 and 94.1 %), Vibrio natriegens (99.4 and 88.8 %), Vibrio parahaemolyticus (99.4 and 90.3 %), Vibrio rotiferianus (99.2 and 94.4 %), Vibrio alginolyticus (99.1 and 89.3 %) and Vibrio campbellii (99.1 and 92.3 %). DNA–DNA hybridization confirmed that the six isolates constitute a unique taxon that is distinct from other known species of Vibrio. In addition, this taxon can be readily differentiated phenotypically from other Vibrio species. The six isolates therefore represent a novel species, for which the name Vibrio jasicida sp. nov. is proposed; the novel species is represented by the type strain TCFB 0772T ( = JCM 16453T = LMG 25398T) (DNA G+C content 45.9 mol%) and reference strains TCFB 1977 ( = JCM 16454) and TCFB 1000 ( = JCM 16455).