Fabrication of a copper nanoparticle/chitosan/carbon nanotube-modified glassy carbon electrode for electrochemical sensing of hydrogen peroxide and glucose

• Published in: Mikrochimica acta (1966) Vol. 160; no. 1-2; pp. 253 - 260
• Main Authors: Wang, Ying, Wei, Wanzhi, Zeng, Jinxiang, Liu, Xiaoying, Zeng, Xiandong
• Format: Journal Article
• Language: English
• Published: Vienna Springer-Verlag 01.01.2008; Springer
• Subjects: Analytical Chemistry; Biological and medical sciences; Biosensors; Biotechnology; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatographic methods and physical methods associated with chromatography; Electrochemical methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Gas chromatographic methods; General, instrumentation; Methods. Procedures. Technologies; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Various methods and equipments; Keywords: Copper; carbon nanotubes; chitosan; glucose oxidase; biosensor; Glucose oxidase; Hydrogen peroxide; Electrochemical method; Nanoparticle; Immobilization; Carbon nanotubes; Selectivity; Electrocatalysis; Efficiency; Detection limit; Biopolymer; Amperometry; Copper; High performance; Hydrogen electrode; Enzyme; Interference; Carbon electrode; Chemical sensor; Gel permeation chromatography; Gas chromatography; Sensitivity; Response time; Biosensor; Oxidoreductases; Chitosan; Enzymatic reaction
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-007-0844-6

. A high-performance amperometric glucose biosensor was developed, based on immobilization of glucose oxidase (GOx) on a copper (Cu) nanoparticles/chitosan (CHIT)/carbon nanotube (CNT)-modified glassy carbon (GC) electrode. The Cu and CNT had a synergistic electrocatalytic effect toward the reduction of hydrogen peroxide in the matrix of biopolymer CHIT. The Cu/CHIT/CNT modified GC electrode could amplify the reduction current of hydrogen peroxide greatly. Besides, the Cu/CHIT/CNT modified GC electrode reduces hydrogen peroxide at a much lower applied potential and inhibit the responses of interferents. With GOx as an enzyme model, a new glucose biosensor was fabricated. The sensitivity of the sensor is due not only to the large microscopic area but also to the high efficiency of transformation of H 2 O 2 generated by enzymatic reaction to current signal. The biosensor exhibited excellent sensitivity (the detection limit is down to 0.02 mM), fast response time (less than 4 sec), wide linear range (from 0.05 to 12 mM), and perfect selectivity.