Bursin as an adjuvant is a potent enhancer of immune response in mice immunized with the JEV subunit vaccine

• Published in: Veterinary immunology and immunopathology Vol. 122; no. 3; pp. 265 - 274
• Main Authors: Wang, Chen, Wen, Wen-yan, Su, Chun-xia, Ge, Fei-fei, Dang, Zhan-guo, Duan, Xiang-guo, Cao, Rui-bing, Zhou, Bing, Chen, Pu-yan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.04.2008; Amsterdam: Elsevier
• Subjects: Adjuvant; Adjuvants, Immunologic; animal models; Animals; antibodies; Antibodies, Viral - blood; Bursin; Cell Proliferation; cytokines; Escherichia coli; Female; helper T cells; immune response; Immunoglobulin G - blood; Immunoglobulin G - immunology; interferons; Japanese Encephalitis Vaccines - administration & dosage; Japanese Encephalitis Vaccines - immunology; Japanese encephalitis virus; JEV; lymphocyte proliferation; Lymphocytes - physiology; Mice; Neutralization Tests; Oligopeptides - immunology; Peptide; Protein Subunits; recombinant fusion proteins; Recombinant Proteins - chemistry; Recombinant Proteins - immunology; Spleen - cytology; splenocytes; synthetic peptides; T-lymphocytes; tripeptides; vaccination; vaccine adjuvants; vaccine development; PBS; BF; HSP; KLH; HRP; TMB; IFE; E-BD; MTT; PRNT; SOE-PCR; BS; FITC; FBS; JEV; Adjuvant; JE; TRX; Peptide; PFU; Bursin
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2007.11.010

Adjuvants play an important role in the formulation of effective and appropriate vaccines. A series of synthetic bursin and bursin-like peptides was heterologously expressed in Escherichia coli. The use of bursin as an adjuvant enhanced the specific immune responses of mice immunized with a recombinant Japanese encephalitis virus E-binding domain (JEV E-BD) fusion protein. The effect was determined in the form of protective anti-JEV E titers, antibodies (IgG1 and IgG2a), spleen cell lymphocyte proliferation, the levels of interferon-γ and interleukin-4 cytokines, and the T-lymphocyte sub-type composition. The IgG2a titer and interferon-γ level suggested that the E-BD protein potentiates the Th1 immune response. These responses were changed when the immunogen was combined with one of the synthetic peptides as adjuvant. JEV-neutralization assay results show that the presence of bursin significantly enhance the JEV-neutralizing titer. We conclude that bursin as an adjuvant is a potent enhancer of immune response in mice immunized with the JEV subunit vaccine, and represents a promising adjuvant for vaccination.