Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples

In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque ® separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were use...

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Published inVeterinary immunology and immunopathology Vol. 58; no. 3; pp. 231 - 237
Main Authors Akens, Margarete K., Holznagel, E., Franchini, M., Bracher, Verena
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 19.09.1997
Subjects
Animals
blood
comparisons
cytolysis
Equine
evaluation
Female
Flow Cytometry
fluorescent antibody technique
horses
Horses - immunology
immune system
Immunophenotyping
Lymphocyte Subsets - immunology
Lymphocytes
Male
monoclonal antibodies
sample processing
separation
PBS
Flow cytometry
BSA
Lymphocytes
DTAF
Immunophenotyping
MHC
Equine
EDTA
mAb
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Summary:In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque ® separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods.
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ISSN:0165-2427
1873-2534
DOI:10.1016/S0165-2427(97)00044-5