Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples
In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque ® separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were use...
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Published in | Veterinary immunology and immunopathology Vol. 58; no. 3; pp. 231 - 237 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
19.09.1997
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Subjects | |
Online Access | Get full text |
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Summary: | In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque
® separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0165-2427 1873-2534 |
DOI: | 10.1016/S0165-2427(97)00044-5 |