Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus

• Published in: Veterinary immunology and immunopathology Vol. 125; no. 3; pp. 284 - 290
• Main Authors: Wang, Shwu-Jinng, Chang, Wei-Feng, Wang, Min-Ying, Hsiung, Kuang-Pin, Liu, Yung-Chuan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.10.2008; Amsterdam: Elsevier
• Subjects: absorbance; accuracy; analytical kits; Animals; Antibodies, Viral - blood; antibody detection; Birnaviridae Infections - diagnosis; Birnaviridae Infections - immunology; Birnaviridae Infections - veterinary; Birnaviridae Infections - virology; Chickens; color; Coloring Agents - chemistry; differential staining; disease detection; Disperse dyes; dye immunochromatographic test; dyes; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; immunoassays; Immunochromatographic test; infectious bursal disease; Infectious bursal disease virus; Infectious bursal disease virus - immunology; Point of need testing (PONT); point-of-need testing; Poultry Diseases - diagnosis; Poultry Diseases - immunology; Poultry Diseases - virology; rapid methods; Reagent Strips - chemistry; screening; Sensitivity and Specificity; serodiagnosis; validity; Disperse dyes; Infectious bursal disease virus; Enzyme-linked immunosorbent assay; Immunochromatographic test; Point of need testing (PONT)

For investigating the feasibility of using disperse dyes as an immunoassay chromogenic marker, a disperse dye, DADISPERSE NAVY BLUE SP, was selected in analyzing antibody against infectious bursal disease virus (anti-IBDV). With the color intensity revealed in the disperse dye immunochromatographic...