Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus

• Published in: Veterinary immunology and immunopathology Vol. 125; no. 3; pp. 284 - 290
• Main Authors: Wang, Shwu-Jinng, Chang, Wei-Feng, Wang, Min-Ying, Hsiung, Kuang-Pin, Liu, Yung-Chuan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.10.2008; Amsterdam: Elsevier
• Subjects: absorbance; accuracy; analytical kits; Animals; Antibodies, Viral - blood; antibody detection; Birnaviridae Infections - diagnosis; Birnaviridae Infections - immunology; Birnaviridae Infections - veterinary; Birnaviridae Infections - virology; Chickens; color; Coloring Agents - chemistry; differential staining; disease detection; Disperse dyes; dye immunochromatographic test; dyes; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; immunoassays; Immunochromatographic test; infectious bursal disease; Infectious bursal disease virus; Infectious bursal disease virus - immunology; Point of need testing (PONT); point-of-need testing; Poultry Diseases - diagnosis; Poultry Diseases - immunology; Poultry Diseases - virology; rapid methods; Reagent Strips - chemistry; screening; Sensitivity and Specificity; serodiagnosis; validity; Disperse dyes; Infectious bursal disease virus; Enzyme-linked immunosorbent assay; Immunochromatographic test; Point of need testing (PONT)
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2008.05.022

For investigating the feasibility of using disperse dyes as an immunoassay chromogenic marker, a disperse dye, DADISPERSE NAVY BLUE SP, was selected in analyzing antibody against infectious bursal disease virus (anti-IBDV). With the color intensity revealed in the disperse dye immunochromatographic test (DICT) strip as the objective function, the optimal dyeing conditions were found as follows: dye concentration absorbance (at λ max = 587 nm) = 3, pH 7, 50 °C, for 10 min. Under these conditions, the resultant dyed-antibody (rabbit anti-chicken) can produce an optimal color intensity reading of 55,054 on the strip. For performing qualitative immunoassay, chicken sera samples taken from different farms were used for the anti-IBDV titre assessment. The results of DICT strips showed very high sensitivity and specificity as compared to that analyzed by FlockChek enzyme linked immunosorbent assay (F-ELISA) kits. For quantitative immunoassay, it was found that the color intensity measured with DICT was linearly correlated to that of F-ELISA titre ( r 2 = 0.9687). Therefore, DICT was further applied to the detection of chicken anti-IBDV sera under vaccination in the farms. The average titres of the sampling groups exhibited a strong agreement to that of F-ELISA. Accordingly, the DICT method developed in this study, shown to be reliable, cheap and simple in both qualitative and quantitative immunoassays, is particularly suitable for point-of-need testing (PONT) in agricultural applications.