Induction of insulin secretion by a component of Urtica dioica leave extract in perifused Islets of Langerhans and its in vivo effects in normal and streptozotocin diabetic rats

• Published in: Journal of ethnopharmacology Vol. 89; no. 1; pp. 47 - 53
• Main Authors: Farzami, Bijan, Ahmadvand, D, Vardasbi, S, Majin, F.J, Khaghani, Sh
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.11.2003; Elsevier
• Subjects: active ingredients; Animals; Biological and medical sciences; blood glucose; Blood Glucose - metabolism; blood serum; Chromatography, Thin Layer; Diabetes; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - physiopathology; General pharmacology; glucose; glycemic effect; Hypoglycemic activity; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; In Vitro Techniques; in vivo studies; Injections, Intraperitoneal; insulin; Insulin - blood; Insulin - metabolism; Insulin Secretion; Islets of Langerhans; Islets of Langerhans - metabolism; leaves; Male; Medical sciences; medicinal plants; Perifusion; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Leaves; Rats; Rats, Wistar; Streptozocin; Streptozotocin; Urtica dioica; Urtica dioica; Hypoglycemic activity; Islets of Langerhans; Diabetes; Perifusion; Streptozotocin; Insulin secretion; Antineoplastic agent; Endocrinopathy; Rat; Medicinal plant; Urticaceae; Hypoglycemic agent; Dicotyledones; Angiospermae; Phytotherapy; Mechanism of action; Pancreas; Pharmacognosy; Secretion; Diabetes mellitus; Rodentia; Langerhans islet; In vitro; Insulin; Biological activity; In vivo; Antibiotic; Vertebrata; Mammalia; Treatment; Animal; Plant origin; Spermatophyta
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/S0378-8741(03)00220-4

The blood glucose lowering effect of Urtica dioica (Stinging Nettle) as a medicinal plant has been noted in old writings such as those of Avicenna. Recently, there has also been other investigators that indicated the hypoglycemic effect of Urtica dioica. But so far, the mechanism of this effect has not been deduced. In this report, a perifusion system is arranged in which an exact number of Langerhans Islets were exposed to several fractions of extracts of Urtica dioica by TLC. The active ingredient fraction named F 1, caused a marked increase in insulin secretion. A simultaneous assay of glucose showed that the increase in insulin level was associated with a decrease in glucose level. Furthermore, the active component of Urtica dioica was found to increase the insulin content of blood sera in normal and streptozotocin diabetic rats that were injected intraperitoneally (i.p.) with the active ingredient of the extract. The in vivo studies presented in this report show that not only an increase in insulin level of blood sera was observed in rats after 30 min from the initial point of injection but a simultaneous decrease of blood sugar was detected when similar sera was tested for glucose. The increase in insulin level was six times during the 120 min of our determination. The decrease in blood sugar was found to be similar both in the level and time of initiation. On the basis of our findings, we assume that F 1 is the active ingredient of plant leaves extract. The results show that the blood lowering effect of the extract was due to the enhancement of insulin secretion by Langerhance Isletes.