Nucleotide sequence of a Drosophila melanogaster cDNA encoding a calnexin homologue

• Published in: Gene Vol. 191; no. 2; pp. 143 - 148
• Main Authors: Christodoulou, S., Lockyer, A.E., Foster, J.M., Hoheisel, J.D., Roberts, D.B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 03.06.1997
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; Base Sequence; Blotting, Northern; Calcium binding; calcium-binding proteins; Calcium-Binding Proteins - chemistry; Calcium-Binding Proteins - genetics; Calnexin; Calreticulin; cell membranes; Chaperone; Chromosome Mapping; Cloning, Molecular; complementary DNA; Conserved Sequence; DNA, Complementary; Drosophila melanogaster; Drosophila melanogaster - genetics; Endoplasmic reticulum; genbank/x99488; gene expression; Gene Expression Regulation, Developmental; Glycosylation; In Situ Hybridization; Insect Proteins - chemistry; Insect Proteins - genetics; messenger RNA; Molecular Chaperones - chemistry; Molecular Sequence Data; nucleotide sequences; Polymerase Chain Reaction; polytene chromosomes; Repetitive Sequences, Nucleic Acid; Ribonucleoproteins - chemistry; Ribonucleoproteins - genetics; RNA, Messenger - analysis; aa, amino acid(s); BSA, bovine serum albumin; oligo, oligodeoxyribonucleotide; UTR, untranslated region(s); RT-PCR, reverse transcriptase-polymerase chain reaction; Chaperone; ORF, open reading frame; kb, kilobase(s) or 1000 bp; Sc., Schizosaccharomyces; Glycosylation; bp, base pair(s); Dm, Drosophila melanogaster; S., Saccharomyces; Calcium binding; Clr, Dm cDNA encoding calreticulin; nt, nucleotide(s); A., Arabidopsis; Cnx, calnexin(s); Endoplasmic reticulum; ER, endoplasmic reticulum; Cnx, Dm cDNA encoding a calnexin-like protein
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(97)00025-5

A cDNA which encodes a calnexin (Cnx)-like protein from Drosophila melanogaster has been characterized. The deduced amino acid sequence shares several regions of homology with Cnx from other sources with two conserved motifs each repeated four times. The gene was found to be transcribed in various tissues and at all developmental stages. We have mapped the gene at chromosomal position 99A and we have also mapped the related gene coding for Drosophila calreticulin at 85E.