Electrochemically modulated liquid chromatographic separation of triazines and the effect of pH on retention

• Published in: Journal of Chromatography A Vol. 1217; no. 26; pp. 4395 - 4401
• Main Authors: Yakes, Betsy Jean, Keller, David W., Porter, Marc D.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25.06.2010; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Analytical chemistry; Applied potential ( Eapp); Biological and medical sciences; Chemical control; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; Electrochemically modulated liquid chromatography (EMLC); Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Herbicide; Herbicides - analysis; Herbicides - isolation & purification; Hydrogen-Ion Concentration; Other chromatographic methods; Parasitic plants. Weeds; Phytopathology. Animal pests. Plant and forest protection; Solvents - chemistry; Triazine; Triazines - analysis; Triazines - isolation & purification; Weeds; Electrochemically modulated liquid chromatography (EMLC); pH; Applied potential ( E app ); Triazine; Herbicide; Chromatographic retention; Pesticides; ); Liquid chromatography; Mobile phase; Ultraviolet detector; Electrochemical gradient; Triazine derivatives; Applied potential (E
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2010.04.031

Electrochemically modulated liquid chromatography (EMLC) manipulates analyte retention by changing the potential applied ( E app ) to a conductive stationary phase. This paper applies EMLC to the separation of a set of seven triazines which are commonly used but environmentally hazardous herbicides. Experiments herein examine the influence of E app and the pH of the mobile phase on triazine retention. The results are discussed in terms of: (1) retention of triazines of dissimilar acid strengths and by correlations with the pH of the mobile phase; (2) how changes in E app and acid–base equilibria modulate elution; (3) qualitative insights into EMLC-based retention; and (4) potential merits of EMLC in realizing the rapid separation of the seven-component triazine mixture.