Effect of recombinant IL-1 beta and recombinant gamma interferon on septic acute lung injury in mice

• Published in: Chest Vol. 105; no. 4; p. 1241
• Main Authors: Torre, D, Minoja, G, Maraggia, D, Chiaranda, M, Tambini, R, Speranza, F, Giola, M
• Format: Journal Article
• Language: English
• Published: United States 01.04.1994
• Subjects: Animals; Escherichia coli; Female; Interferon-gamma - administration & dosage; Interleukin-1 - administration & dosage; Lipopolysaccharides; Lung - pathology; Mice; Mice, Inbred Strains; Neutrophils - pathology; Pulmonary Edema - complications; Pulmonary Edema - pathology; Recombinant Proteins; Respiratory Distress Syndrome, Adult - complications; Respiratory Distress Syndrome, Adult - pathology; Respiratory Distress Syndrome, Adult - prevention & control
• ISSN: 0012-3692
• DOI: 10.1378/chest.105.4.1241

The effect of recombinant human interleukin 1B (IL-1B) and recombinant human gamma interferon (IFN-g), when given prophylactically, in a mouse model of septic acute lung injury was studied. Mice were treated with various doses of IL-1B and IFN-g for 3 consecutive days prior to administration of lipopolysaccharide of Escherichia coli (1 mg/kg given intraperitoneally). To determine the histologic changes occurring after prophylactic administration of such cytokines, a scoring system was assessed. A significant reduction of edema and neutrophil accumulation into the lungs of mice was observed, especially at doses of 100 U per mouse and 10,000 U per mouse of IL-1B and IFN-g, respectively. Prophylactic administration of IL-1B or IFN-g caused histologic changes, including marked reduction of edema and neutrophil accumulation in the interstitial and alveolar spaces. Combined prophylactic administration of IL-1B and IFN-g provoked a marked decrease of neutrophil accumulation into the lungs, but was not accompanied by significant reduction of edema or hemorrhage. These results provide evidence for the beneficial role of IL-1B and IFN-g in the abnormality of septic acute lung injury by reducing inflammatory lesions.