A large open reading frame (orf1995) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein
An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is hig...
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Published in | Molecular & general genetics Vol. 253; no. 5; pp. 649 - 653 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
20.02.1997
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Subjects | |
Online Access | Get full text |
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Summary: | An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival. |
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Bibliography: | F60 1997B10578 F30 |
ISSN: | 0026-8925 1432-1874 |
DOI: | 10.1007/s004380050368 |