D-Methionine Attenuates Inner Hair Cell Loss in Carboplatin-Treated Chinchillas

• Published in: Audiology & neurotology Vol. 5; no. 5; pp. 263 - 266
• Main Authors: Lockwood, D.S., Ding, D.L., Wang, J., Salvi, R.J.
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.09.2000; S. Karger AG
• Subjects: Animals; Antineoplastic Agents - adverse effects; Biological and medical sciences; Carboplatin - adverse effects; Chinchilla; Drug toxicity and drugs side effects treatment; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology; Hair Cells, Auditory, Inner - drug effects; Medical sciences; Methionine - pharmacology; Non tumoral diseases; Original Paper; Otorhinolaryngology. Stomatology; Pharmacology. Drug treatments; Rodentia; Toxicity: respiratory system, ent, stomatology; Carboplatin; D-Methionine; Hair cells; Chinchilla; Ototoxicity; Antineoplastic agent; Toxicity; Auditory disorder; Inner ear; Prevention; Secondary effect; ENT disease; Protection; Platinum II Complexes; Enzyme; Ear disease; Internal ear disease; Transferases; Treatment efficiency; Rodentia; Cochleography; Exploration; Experimental study; Vertebrata; D-Methionine-pyruvate transaminase; Mammalia; Treatment; Transaminases; Adult animal; Hair cell(ear); Comparative study
• ISSN: 1420-3030; 1421-9700
• DOI: 10.1159/000013890

Carboplatin, a second-generation platinum-based antineoplastic drug, preferentially destroys inner hair cells (IHCs) in the chinchilla while sparing outer hair cells (OHCs). D-Methionine (D-Met), a sulfur-containing amino acid, has been shown to protect hair cells from cisplatin damage in rats, but its ability to protect IHCs from carboplatin damage has not yet been evaluated in the chinchilla. We tested whether D-Met would protect the hair cells in the chinchilla from carboplatin. Animals were divided into two groups: a control group that only received carboplatin (100 mg/kg, i.p.) and an experimental group that received 300 mg/kg D-Met (i.p.) 30 min before carboplatin treatment. Ototoxicity was assessed by measuring the amount of IHC and OHC loss. Average IHC loss in the group treated with D-Met was 62% compared with 84% in the untreated control group. Thus, D-Met causes a statistically significant reduction in IHC loss induced by carboplatin.