Enhanced production of laccase by Coriolus hirsutus using molasses distillery wastewater

• Published in: Frontiers of environmental science & engineering Vol. 7; no. 2; pp. 200 - 210
• Main Authors: Sun, Wei, Xu, Meiying, Xia, Chunyu, Li, Anhua, Sun, Guoping
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.04.2013; Higher Education Press; SP Higher Education Press; Springer Nature B.V
• Subjects: Agitation; Chemical oxygen demand; confidence interval; Coriolus hirsutus; Cultivation; Decoloring; Decolorization; Dilution; Distilleries; Earth and Environmental Science; Environment; Fractional factorial design; incubation period; Inoculation; Inoculum; Laccase; Mathematical models; Molasses; molasses distillery wastewater; nitrogen; Parameters; Physical properties; Research Article; response surface method; Response surface methodology; Statistical analysis; Syrups & sweeteners; temperature; Wastewater; 中心复合设计; 云芝; 介壳虫; 实验数据; 搅拌速率; 漆酶; 生产; 糖蜜酒精废水; molasses distillery wastewater; response surface method; laccase; fractional factorial design
• ISSN: 2095-2201; 2095-221X
• DOI: 10.1007/s11783-012-0457-0

The effect of physical parameters of cultivation （load volume, temperature, pH, agitation, inoculum size, and incubation period） in the production of laccase by wood-rotting basidiomycete Coriolus hirsutus were stu- died using diluted molasses distillery wastewater （MDW） as a major composition. Using fractional factorial design, our study first identified load volume, agitation, and inoculum size as statistically significant factors. Optimal preferences and mutual interactions of the factors were then determined by the response surface method, which is based on the center composite design. A quadratic model was used to fit the experimental data. The optimized operational parameters for laccase production were determined to be the following： culture temperature of 25~C, pH 4, load volume of 40 mL diluted MDW in 150 mL flask, agitation rate of 183 r-m in-~, inoculation of 11.5% v/v, and cultivation time of 6 d. The experimental validation under these conditions （the maximum laccase production of 2198.2U.mL-1 was within the confidence interval） subsequently verified the accuracy of the constructed model. Moreover, the removal of chemical oxygen demand and nitrogen of MDW reached 62.85% and 48.00% respectively, and the decolorization ratio under the optimal condition was 41.85%. The enhanced production of laccase by C. hirsutus is a new recovery strategy for MDW.