Assessment of Chilling Injury and Molecular Marker Analysis in Cucumber Cultivars (Cucumis sativus L.)

• Published in: Weon'ye gwahag gi'sulji Vol. 32; no. 2; pp. 227 - 234
• Main Authors: Ali, Asjad, Yang, Eun Mi, Bang, Sun Woong, Chung, Sang-Min, Staub, Jack E.
• Format: Journal Article
• Language: English
• Published: 한국원예학회HST 2014; 한국원예학회
• Subjects: 농학; chloroplast genotypes; low temperature; abiotic stress; DNA marker; sdCAPS
• ISSN: 1226-8763; 2465-8588
• DOI: 10.7235/hort.2014.13138

The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previouslydetermined cold tolerant NC76 and ‘Chipper’), and Chinese and Japanese germplasms. Seedlings of each entry were exposedto 4°C (Experiment 1) and 1°C (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under80% relative humidity (RH) and 149 μmoles･m-2･s-1photosynthetic photon flux (PPF). The chilling response [damage rating(DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate,4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and ‘Chipper’ afterchilling w as 1 a nd 1 .1, respectively . Korean ‘Nacdongchungjang’ w as m ost sensitive to c hilling temperatures [DR = 2 .3] whencompared to the other entries examined. The sensitivity to chilling of ‘Nacdongchungjang’ was followed by Chinese ‘Dongguan’[DR = 1.7]. In contrast, ‘Saeronchungjang’ (DR = 1) and ‘Janghyungnachap’ (DR = 1) were the most chilling tolerant of the Koreanaccessions examined and equivalent to the response of line NC76 and ‘Chipper’. Nevertheless, chloroplast type genotyping ofthese accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerantlines (NC76 and ‘Chipper’) and all Korean lines examined. KCI Citation Count: 4