Determination of oxytetracycline in milk samples by polymer inclusion membrane separation coupled to high performance liquid chromatography

• Published in: Analytica chimica acta Vol. 718; pp. 42 - 46
• Main Authors: Pérez-Silva, Irma, Rodríguez, José A., Ramírez-Silva, Ma. Teresa, Páez-Hernández, Ma. Elena
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 09.03.2012; Elsevier
• Subjects: Analytical chemistry; Animals; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - isolation & purification; Cellulose - analogs & derivatives; Cellulose - chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Cyanex® 923; Ethers; Exact sciences and technology; High performance liquid chromatography; Inclusions; Limit of Detection; Mathematical analysis; Membranes; Membranes, Artificial; Milk; Milk - chemistry; Optimization; Other chromatographic methods; Oxytetracycline; Oxytetracycline - analysis; Oxytetracycline - isolation & purification; Plackett–Burman experimental design; Polymer inclusion membrane; Polymers - chemistry; Oxytetracycline; Plackett–Burman experimental design; Cyanex® 923; Polymer inclusion membrane; Milk; Ether; Sample; HPLC chromatography; Polymer; Selectivity; Method; Membrane separation; Plasticizer; Experimental design; Detection limit; Linearity; Membrane; 923; Cellulose acetate; Cyanex; Quantitative analysis; Plackett-Burman experimental design
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2011.12.057

[Display omitted] ► Selective extraction and HPLC analysis of oxytetracycline (OTC) in milk samples. ► Preparation and characterization of a polymer inclusion membrane (PIM). ► Optimization of PIM–HPLC method using a Plackett–Burman experimental design. ► The OTC was isolated and the matrix interferences were eliminated. ► Limit of detection useful for OTC residues analysis in real milk samples (8ngmL−1). The determination of oxytetracycline in milk samples using a polymer inclusion membrane concept with high performance liquid chromatography (HPLC) was studied. The membranes developed are composed by cellulose acetate as polymer base, Cyanex 923 as carrier and o-nitrophenyl octyl ether as plasticizer. In the optimal conditions, the method exhibits good linearity in the range 0.03–0.20mgL−1 with a limit of detection and quantification of 8.2 and 27.3μgL−1 respectively. The method was successfully applied to the analysis of milk samples with high selectivity.