Molecularly imprinted polyaniline molecular receptor–based chemical sensor for the electrochemical determination of melamine

• Published in: Journal of molecular recognition Vol. 33; no. 7; pp. e2836 - n/a
• Main Authors: Regasa, Melkamu B., Soreta, Tesfaye R., Femi, Olu E., Ramamurthy, Praveen C., Kumar, Saravana
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.07.2020
• Subjects: Aniline; Baby foods; Casein; Chemical sensors; Conducting polymers; electrochemical sensor; Electrochemistry; Glassy carbon; glassy carbon electrode; Glycine; Histidine; imprinted polyaniline; Imprinted polymers; Melamine; Polyanilines; Polymerization; Polymers; Recyclability; Selectivity; Sensors; Square waves; Voltammetry; glassy carbon electrode; imprinted polyaniline; melamine; electrochemical sensor
• ISSN: 0952-3499; 1099-1352
• DOI: 10.1002/jmr.2836

Molecularly imprinted polymer‐modified glassy carbon electrode (GCE)‐based electrochemical sensor is prepared using the electropolymerization of aniline in the presence of melamine (MA) as a template. In this work, the advantages of molecularly imprinted conducting polymers (MICPs) and electroanalytical methods were combined to obtain an electronic device with better performances. The sensor performance was evaluated by cyclic voltammetry (CV) and square wave voltammetry (SWV) with the linear range of 0.6‐16 × 10−9M, quantification limit of 14.9 × 10−10M, and detection limit of 4.47 × 10−10M (S/N = 3). The selectivity of the sensor was tested in the presence of acetoguanamine (AGA), diaminomethylatrazine (DMT), casein, histidine, and glycine interfering molecules taken at the triple concentration with MA that demonstrated too small current response compared with that of the analyte indicating high specificity of the sensor towards the template. The sensor was successfully applied to determine MA in infant formula samples with significant recovery greater than 96% and relative standard deviation (RSD) less than 4.8%. Moreover, the good repeatability, recyclability, and stability make this sensor device promising for the real‐time monitoring of MA in different food stuffs. Thin MIP films–based electrochemical sensors for detection of melamine is developed. The MIP sensor is based on the polyaniline of conducting polymer. The polymer film is prepared by potentiodynamic electropolymerization method in the presence of melamine as a template. The rebinding of the template molecule was confirmed by SWV using ferricyanide labelling probe. The polymer film responds to melamine molecule at nano‐level concentration.