bFGF induces its own gene expression in astrocytic and hippocampal cell cultures

Basic FGF mRNA induction by bFGF was investigated in cell cultures from rat brain, i.e. postnatal day 2 cortex and embryonic day 18 hippocampus. In situ hybridization shows that after bFGF treatment (10(-10) M) for 14 h neurones and glial cells show a remarkable increase in bFGF mRNA production. Inc...

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Bibliographic Details
Published inNeuroreport Vol. 3; no. 12; p. 1077
Main Authors Flott-Rahmel, B, Gerdes, W, Pechan, P A, Brysch, W, Schlingensiepen, K H, Seifert, W
Format Journal Article
LanguageEnglish
Published England 01.12.1992
Subjects
Animals
Animals, Newborn
Astrocytes - drug effects
Astrocytes - metabolism
Cell Division - drug effects
Cells, Cultured
Digoxigenin
Fibroblast Growth Factor 2 - genetics
Fibroblast Growth Factor 2 - pharmacology
Gene Expression - drug effects
Hippocampus - drug effects
Hippocampus - metabolism
In Situ Hybridization
Rats
RNA Probes
RNA, Antisense - metabolism
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Summary:Basic FGF mRNA induction by bFGF was investigated in cell cultures from rat brain, i.e. postnatal day 2 cortex and embryonic day 18 hippocampus. In situ hybridization shows that after bFGF treatment (10(-10) M) for 14 h neurones and glial cells show a remarkable increase in bFGF mRNA production. Incubation of astrocytes with antisense bFGF phosphorothioate oligodeoxynucleotides (bFGF-PTOs) resulted in an inhibition of both bFGF induced and serum induced proliferation. The results indicate that bFGF is capable of inducing its own mRNA production. This induction, i.e. new synthesis of bFGF mRNA, seems to be essential for the mitogenic effect of both bFGF and serum components.
ISSN:0959-4965
DOI:10.1097/00001756-199212000-00011