Dynamic Phaeodactylum tricornutum exometabolites shape surrounding bacterial communities

• Published in: The New phytologist Vol. 239; no. 4; pp. 1420 - 1433
• Main Authors: Brisson, Vanessa, Swink, Courtney, Kimbrel, Jeffrey, Mayali, Xavier, Samo, Ty, Kosina, Suzanne M., Thelen, Michael, Northen, Trent R., Stuart, Rhona K.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.08.2023; Wiley-Blackwell
• Subjects: 4‐hydroxybenzoic acid; Abundance; Algae; Algal growth; algal–bacterial interactions; Bacteria; Bacteria - metabolism; Chromatography; Chromatography, Liquid; Community composition; Composition; Diatoms; Diatoms - metabolism; exometabolites; Exudates; Exudation; Germfree; Liquid chromatography; lumichrome; Mass Spectrometry; Mass spectroscopy; Metabolites; microbiome; Microorganisms; p-Hydroxybenzoic acid; Phaeodactylum tricornutum; Secretion; Substrates; algal-bacterial interactions; lumichrome; Phaeodactylum tricornutum; 4-hydroxybenzoic acid; microbiome; exometabolites
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.19051

Summary Roles of different ecological classes of algal exometabolites in regulating microbial community composition are not well understood. Here, we identify exometabolites from the model diatom Phaeodactylum tricornutum and demonstrate their potential to influence bacterial abundances. We profiled exometabolites across a time course of axenic algal growth using liquid chromatography–tandem mass spectrometry. We then investigated growth of 12 bacterial isolates on individual‐identified exometabolites. Lastly, we compared responses of a P. tricornutum‐adapted enrichment community to additions of two contrasting metabolites: selective growth substrate 4‐hydroxybenzoic acid and putative signaling/facilitator molecule lumichrome. We identified 50 P. tricornutum metabolites and found distinct temporal accumulation patterns. Two exometabolites (of 12 tested) supported growth of distinct subsets of bacterial isolates. While algal exudates and algal presence drove similar changes in community composition compared with controls, exogenous 4‐hydroxybenzoic acid addition promoted increased abundances of taxa that utilized it in isolation, and also revealed the importance of factors relating to algal presence in regulating community composition. This work demonstrates that secretion of selective bacterial growth substrates represents one mechanism by which algal exometabolites can influence bacterial community composition and illustrates how the algal exometabolome has the potential to modulate bacterial communities as a function of algal growth.