Role of thymic B cells in the development of thymus-derived regulatory T cell in vitro

• Published in: Immunology letters Vol. 185; pp. 56 - 63
• Main Authors: Muhammed Ali, Haider H, Muhammed Ali, Adnan H, Drela, Nadzieja
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2017
• Subjects: Allergy and Immunology; Aminoquinolines - immunology; Animals; Antigens, CD - metabolism; B-Lymphocytes - immunology; Cell Differentiation; Cells, Cultured; Costimulatory molecules; Forkhead Transcription Factors - genetics; Forkhead Transcription Factors - metabolism; Histocompatibility Antigens Class II - metabolism; IMQ; Lipopolysaccharides - immunology; LPS; Lymphocyte Activation; Male; MHC II; Mice; Mice, Inbred C57BL; Mice, Knockout; nTregs; T-Lymphocyte Subsets - immunology; T-Lymphocytes, Regulatory - immunology; Thymic B cells; Thymocytes - immunology; Thymus Gland - immunology; Thymic B cells; MHC II; IMQ; LPS; costimulatory molecules; nTregs; Costimulatory molecules
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/j.imlet.2017.03.007

Highlights • Thymic B cells in the condition of our experiments did not influence the generation of nTregs, but rather maintain their viability when activated by both activators. • Activated thymic B cells maintained the high percentage of nTreg generation or counteracted the decrease of this percentage observed in non-activated culture. • Both activators (LPS and IMQ) have the same effect in the process of nTreg generation by B cells. • The percentage of B cells was strongly increased in the conditions of our experiments\72 h in LPS and IMQ-supplemented medium.