Development of microsatellite markers in the Australasian snake-necked turtle Chelodina rugusa and cross-species amplification
Seventeen microsatellite loci were developed for the snake-necked turtle, Chelodina rugosa (Ogilby, 1890). Sixteen of the loci were polymorphic but three of these loci had null alleles. One locus displayed linkage disequilibrium. These 17 markers were tested for amplification in eight congeneric spe...
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Published in | Molecular ecology resources Vol. 9; no. 1; pp. 350 - 353 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.01.2009
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Subjects | |
Online Access | Get full text |
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Summary: | Seventeen microsatellite loci were developed for the snake-necked turtle, Chelodina rugosa (Ogilby, 1890). Sixteen of the loci were polymorphic but three of these loci had null alleles. One locus displayed linkage disequilibrium. These 17 markers were tested for amplification in eight congeneric species with varying success; 98% amplification in Chelodina burrungandjii, 72% in C. canni, 38% in C. expansa, 58% in C. longicollis, 67% in C. mccordi, 73% in C. oblonga, 81% in C. parkeri, and 68% in C. pritchardi. These microsatellite markers will be useful for population assignment, gene flow, mating systems and hybridization studies in the genus Chelodina. |
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Bibliography: | ark:/67375/WNG-DQKKZH34-P ArticleID:MEN2389 istex:0D311378BF8EE68959D9D19AA9ECB8EC40EA924E ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1755-098X 1755-0998 |
DOI: | 10.1111/j.1755-0998.2008.02389.x |