Development of microsatellite markers in the Australasian snake-necked turtle Chelodina rugusa and cross-species amplification

Seventeen microsatellite loci were developed for the snake-necked turtle, Chelodina rugosa (Ogilby, 1890). Sixteen of the loci were polymorphic but three of these loci had null alleles. One locus displayed linkage disequilibrium. These 17 markers were tested for amplification in eight congeneric spe...

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Published inMolecular ecology resources Vol. 9; no. 1; pp. 350 - 353
Main Authors ALACS, E. A, HILLYER, M. J., GEORGES, A., FITZSIMMONS, N. N., HUGHES, J. M.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.01.2009
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Summary:Seventeen microsatellite loci were developed for the snake-necked turtle, Chelodina rugosa (Ogilby, 1890). Sixteen of the loci were polymorphic but three of these loci had null alleles. One locus displayed linkage disequilibrium. These 17 markers were tested for amplification in eight congeneric species with varying success; 98% amplification in Chelodina burrungandjii, 72% in C. canni, 38% in C. expansa, 58% in C. longicollis, 67% in C. mccordi, 73% in C. oblonga, 81% in C. parkeri, and 68% in C. pritchardi. These microsatellite markers will be useful for population assignment, gene flow, mating systems and hybridization studies in the genus Chelodina.
Bibliography:ark:/67375/WNG-DQKKZH34-P
ArticleID:MEN2389
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ISSN:1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2008.02389.x