Distribution of High-risk Human Papillomavirus Genotypes in Cervical Secretions in Punjab

• Published in: Journal of the College of Physicians and Surgeons--Pakistan Vol. 31; no. 7; pp. 786 - 791
• Main Authors: Minhas, Sadia, Kashif, Muhammad, Rehman, Zobaria, Pasha, Muhammad Bilal, Idrees, Muhammad, Ansari, Farheen
• Format: Journal Article
• Language: English
• Published: Pakistan College of Physicians and Surgeons Pakistan 01.07.2021
• Subjects: Alphapapillomavirus; Cancer; Cervix Uteri; Cross-Sectional Studies; DNA, Viral - genetics; Female; Genetic aspects; Genital diseases, Female; Genotype; Humans; Oncology, Experimental; Pakistan - epidemiology; Papillomaviridae - genetics; Papillomavirus infections; Papillomavirus Infections - diagnosis; Papillomavirus Infections - epidemiology; Papillomaviruses; Prevalence; Risk factors; Statistics; Uterine Cervical Neoplasms - epidemiology; Pakistan
• ISSN: 1022-386X; 1681-7168
• DOI: 10.29271/jcpsp.2021.07.786

To determine the high risk-human papillomavirus (HR-HPV) prevalence, genotype distribution, and associated risk factors in women with general gynaecological problems. Descriptive, cross-sectional study. Lady Wallingdon Hospital, Lahore from August 2018 to May 2019. One hundred females with general gynaecological problems constituted the sample. Real-time polymerase chain reaction and liquid base cytology were performed for HR-HPV detection, genotyping, and cytological changes in cervical samples. The overall prevalence of HR-HPV was found to be 57%, with the most frequent HR-HPV genotypes HPV 16 (18%), HPV 18 (6%), and HPV 45 (1%). The total prevalence of single HR-HPV infection was 25%; whereas, multiple co-infection of HR-HPV was seen in 32% of cases. In total, 81% of these women had normal cytology and 19% had cytologic abnormalities. A significant association was observed between HR-HPV infection and general gynaecological complaints and between HR-HPV infection and cytological abnormalities (p < 0.001). The HR-HPV prevalence was 57% with the most prevalent genotypes were HPV 16 and HPV 18. The results will also aid the identification of HR-HPV 51 and 31 genotypes, which should be added in the multi-type HPV vaccines. Key Words: Cancer of cervix, Human papillomavirus, Papanicolaou test, Pakistan.