Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system

• Published in: Stem cell research Vol. 47; p. 101885
• Main Authors: Shan, Yongli, Ma, Lishi, Zhang, Cong, Zhang, Yanqi, Zhang, Jingyuan, Xiao, Lu, Wei, Yanxing, Yu, Yanhong
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.08.2020; Elsevier

Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human...