Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system

Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human...

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Published inStem cell research Vol. 47; p. 101885
Main Authors Shan, Yongli, Ma, Lishi, Zhang, Cong, Zhang, Yanqi, Zhang, Jingyuan, Xiao, Lu, Wei, Yanxing, Yu, Yanhong
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.08.2020
Elsevier
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Summary:Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro.
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ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2020.101885