Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system
Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human...
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| Published in | Stem cell research Vol. 47; p. 101885 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
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England
Elsevier B.V
01.08.2020
Elsevier |
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| Abstract | Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro. |
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| AbstractList | Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro.Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro. Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro. |
| ArticleNumber | 101885 |
| Author | Xiao, Lu Zhang, Cong Shan, Yongli Zhang, Jingyuan Wei, Yanxing Ma, Lishi Zhang, Yanqi Yu, Yanhong |
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| Cites_doi | 10.1126/science.1231143 10.1016/j.cell.2017.08.002 10.1038/s41467-019-14028-x |
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| References | Cong, Ran, Cox, Lin, Barretto, Habib, Hsu, Wu, Jiang, Marraffini (b0005) 2013; 339 Schuettengruber, Bourbon, Di Croce, Cavalli (b0010) 2017; 171 Shan, Zhang, Zhao, Wang, Zhang, Yao, Ma, Liang, Huang, Huang (b0015) 2020; 11 Shan (10.1016/j.scr.2020.101885_b0015) 2020; 11 Cong (10.1016/j.scr.2020.101885_b0005) 2013; 339 Schuettengruber (10.1016/j.scr.2020.101885_b0010) 2017; 171 34864220 - Stem Cell Res. 2021 Dec;57:102604. doi: 10.1016/j.scr.2021.102604. |
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| Title | Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system |
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