Structure and expression of ovine complement receptor type 2

• Published in: Veterinary immunology and immunopathology Vol. 72; no. 1; pp. 67 - 72
• Main Authors: Young, A.J, Dudler, L, Yamaguchi, K, Marston, W, Hein, W.R
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.12.1999
• Subjects: Animals; ANTIGENE; ANTIGENOS; ANTIGENS; B-cell subsets; B-Lymphocyte Subsets - immunology; Cloning, Molecular; Complement; complement receptor 2; DNA - chemistry; FACTEUR IMMUNOLOGIQUE; FACTORES INMUNOLOGICOS; Gene Expression Regulation, Developmental; IMMUNOLOGICAL FACTORS; LINFOCITOS; LYMPHOCYTE; LYMPHOCYTES; OVIN; OVINOS; Precipitin Tests - veterinary; Protein Isoforms - genetics; Protein Isoforms - immunology; Receptor dimerization; Receptors, Complement 3d - chemistry; Receptors, Complement 3d - genetics; Sequence Analysis, Protein; SHEEP; Ubiquitin; Ubiquitin; Receptor dimerization; Complement; B-cell subsets
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/S0165-2427(99)00112-9

The structure of sheep complement receptor type 2 (CR2) was characterised by cDNA cloning, protein sequencing and immunoprecipitation. The primary structure of sheep CR2 is similar to known mammalian homologues but the higher-order structure is unusual. Two distinct CR2 isoforms occur, one of which is ubiquitinated in the cytoplasmic domain, and the two molecular forms are expressed at the cell surface as non-covalently associated dimers. The percentage of sheep B-cells that express CR2 changes during development and varies between different body compartments. CR2 + and CR2 − B-cell subsets also differ in the expression of other surface markers and in functional properties. Differential expression of CR2 may, therefore, delineate B-cells that arose by alternative developmental pathways, or it could be a marker for B-cells at different phases of antigen exposure.