Genetic Structure of Native Blue Honeysuckle Populations in the Western and Eastern Eurasian Ranges

• Published in: Plants (Basel) Vol. 11; no. 11; p. 1480
• Main Authors: Naugžemys, Donatas, Patamsytė, Jolanta, Žilinskaitė, Silva, Hoshino, Yoichiro, Skridaila, Audrius, Žvingila, Donatas
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.05.2022; MDPI
• Subjects: Bayesian analysis; Berries; Chemical composition; Chloroplast DNA; Chloroplasts; Clustering; Cold; Cultivars; Deoxyribonucleic acid; DNA; Genetic analysis; Genetic diversity; Genetic relationship; Genetic structure; Genotypes; Habitats; Industrial development; Lonicera; Lonicera caerulea; Markers; molecular markers; Morphology; Nucleotide sequence; Polymorphism; Population; population genetic structure; Population genetics; Population studies; Populations; Ripening; Sequence analysis; speciation; Taxonomy; Latvia; United States > US; China; Japan; North America; Baltic Sea; Estonia; Russia; polymorphism; population genetic structure; molecular markers; Lonicera; speciation
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants11111480

Blue honeysuckle ( L.) is a promising berry crop producing edible early-ripening berries with a valuable chemical composition. We evaluated the genetic diversity of native populations from the western (Baltic states) and eastern (the Russian Far East and Japan) edges of the Eurasian range using inter-simple sequence repeat (ISSR) and chloroplast DNA ( A- H and L- F) markers. The genetic relationships of populations and genotypes were analyzed using principal coordinate and cluster analyses (neighbor joining and Bayesian clustering). Sampling was carried out in two disjunct areas of this circumpolar species and the analyses showed clustering of individuals and populations according to geographic origin. The analysis of genetic structure based on ISSR markers showed that the studied populations of were highly differentiated. However, sequence analysis of two chloroplast DNA (cpDNA) regions revealed no phylogeographic structure among the populations. We also found that the eastern populations of blue honeysuckle had significantly greater genetic diversity parameters than the populations from the Baltic region. This finding correlates with the endangered status of blue honeysuckle in the Baltic states.