Reproducibility of Nerve Fiber Layer Thickness Measurements Using Optical Coherence Tomography in Silicone Oil-Filled Eyes

• Published in: Ophthalmologica (Basel) Vol. 215; no. 2; pp. 91 - 96
• Main Authors: Mastropasqua, Leonardo, Carpineto, Paolo, Ciancaglini, Marco, Falconio, Gennaro, Harris, Alon
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.03.2001; S. Karger AG
• Subjects: Biological and medical sciences; Case-Control Studies; Diagnostic Techniques, Ophthalmological; Female; Humans; Interferometry; Investigative techniques, diagnostic techniques (general aspects); Light; Male; Medical sciences; Middle Aged; Nerve Fibers - pathology; Ophthalmology; Optic Nerve - pathology; Optic Nerve Diseases - diagnosis; Original Paper · Travail original · Originalarbeit; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Prospective Studies; Reproducibility of Results; Retinal Detachment - surgery; Retinal Ganglion Cells - pathology; Silicone Oils - therapeutic use; Tomography - methods; Vitrectomy; Vitreous Body; Silicone oil tamponade; Vitrectomy; Nerve fiber layer; Optical coherence tomography; Human; Measurement; Evaluation; Radiodiagnosis; Intraocular; Silicone oil; Exploration; Retina; Thickness; Light coherence; Reproducibility; Surgery; Tomography; Nerve fiber
• ISSN: 0030-3755; 1423-0267
• DOI: 10.1159/000050836

Purpose: To evaluate the reproducibility of nerve fiber layer (NFL) thickness measurements by optical coherence tomography (OCT) in individuals with silicone oil-filled eyes. Methods: Eighteen patients who had undergone pars plana vitrectomy and silicone oil tamponade for retinal detachment were enrolled in a prospective, case-controlled clinical study. Each patient underwent OCT measurement of NFL thickness. Five repetitions of a series of scans on five separate occasions within a 0.5-month period were performed. Each eye was scanned at two different nerve head programs [radius (R) = 1.5 and R = 1.73]. The contralateral healthy eye was used as control. For each option (R = 1.5 and R = 1.73) and region (superior, inferior, temporal, nasal and overall mean), variance components and intraclass correlation coefficients (ICCs) were determined using repeated-measures regression. In these models, NFL thickness, as measured by OCT, was considered to have three variance components: intersubject, intervisit (within subject, between dates), and intravisit (within subject, within date). The ICC (intersubject variance/total variance) was used as a measure of reliability. Results: The contralateral healthy eye provided a higher degree of reproducibility than the silicone oil-filled eye (p = 0.0001). Reproducibility was higher in a given eye on a given visit than from visit to visit. Reproducibility as measured by ICCs was as follows: R = 1.5, 0.54/0.30 (control eyes/silicone oil-filled eyes); R = 1.73, 0.51/0.30. Conclusions: Reproducibility results for OCT measurement of NFL thickness are different in healthy eyes and silicone oil-filled eyes. NFL thickness measurement is not reliable in silicone oil-filled eyes.