Prevention of lipopolysaccharide-induced mouse lethality by resveratrol

• Published in: Food and chemical toxicology Vol. 48; no. 6; pp. 1543 - 1549
• Main Authors: Sebai, Hichem, Sani, Mamane, Ghanem-Boughanmi, Néziha, Aouani, Ezzedine
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.2010; Elsevier
• Subjects: Animals; Biological and medical sciences; Catalase - metabolism; Chemical and industrial products toxicology. Toxic occupational diseases; Iron; Iron - blood; Iron - metabolism; Lethality; Lipid Peroxidation - drug effects; Lipopolysaccharide; Lipopolysaccharides - toxicity; Male; Medical sciences; Metals and various inorganic compounds; Mice; Nitric Oxide; Nitric Oxide - blood; Nitric Oxide - metabolism; Oxidative stress; Oxidative Stress - drug effects; Resveratrol; Stilbenes - pharmacology; Toxicology; NO; Oxidative stress; Lethality; Iron; MDA; Nitric Oxide; LPS; RVT; NOS; CAT; ROS; Resveratrol; Lipopolysaccharide; Rodentia; Transition metal; Antioxidant; Stilbene derivatives; Prevention; Vertebrata; Polyphenol; Mammalia; Mouse; Phytoalexin; Animal; Nitric oxide; Phenols
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2010.03.022

The present study was undertaken to determine whether subacute treatment with resveratrol (RVT) protects mice against lipopolysaccharide (LPS)-induced oxidative stress and mortality as well as the mechanism involved in such protection. Mice were divided into three groups: control, LPS and LPS+RVT. Animals were pre-treated with RVT during 7days. The survival rate was monitored over 48h after LPS administration. Survival animals were sacrificed, their kidney, liver and brain homogenized for malondialdehyde (MDA), catalase (CAT) activity, free iron and nitric oxide (NO) determination. Plasma was also processed for transaminases, creatinine, urea, NO and iron measurement. Pre-treatment with resveratrol greatly improved the survival rate of LPS-treated mice. Resveratrol counteracted LPS-induced tissue lipoperoxidation and catalase activity depletion. The polyphenol abrogated LPS-induced liver and kidney dysfunction as increased creatinine and urea as well as transaminases activities. In addition, pre-treatment with resveratrol abrogated LPS-induced tissues and plasma NO elevation and iron sequestration from plasma to tissue compartment. These data suggest that resveratrol prevents LPS-induced lethality and that its mode of action may involve differential iron deposition via iron shuttling proteins.