Glutamine transfer from xylem to phloem and translocation to developing leaves of Populus deltoides

• Published in: Plant physiology (Bethesda) Vol. 77; no. 2; pp. 412 - 417
• Main Authors: Dickson, R.E, Vogelmann, T.C, Larson, P.R
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.02.1985
• Subjects: Absorption. Translocation of ions and substances. Permeability; Agronomy. Soil science and plant productions; Autoradiography; Biological and medical sciences; Cylinders; Economic plant physiology; FEUILLE; FLOEMA; Fundamental and applied biological sciences. Psychology; HOJAS; Internodes; LEAVES; Metabolism; Metabolism. Physicochemical requirements; METABOLISME DE L'AZOTE; METABOLISMO DEL NITROGENO; NITROGEN METABOLISM; Nutrition. Photosynthesis. Respiration. Metabolism; Parenchyma; PHLOEM; PHLOEME; Plant physiology and development; POPULUS DELTOIDES; Radiocarbon; Sap; Secondary xylem; TRANSFERS; TRANSLOCACION; TRANSLOCATION; XILEMA; XYLEM; XYLEME; Translocation; Populus deltoides; Dicotyledones; Angiospermae; Spermatophyta; Conductive tissue; Salicaceae; Glutamine
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.77.2.412

The distribution of 14C from xylem-borne [14C]glutamine, the major nitrogen compound moving in xylem sap of cottonwood (Populus deltoides Bartr. ex Marsh), was followed in rapidly growing shoots with a combination of autoradiographic, microautoradiographic, and radioassay techniques. Autoradiography and 14C analyses of tissues showed that xylemborne glutamine did not move with the transpiration stream into mature leaves. Instead, most of it was transferred from xylem to phloem in the upper stem and then translocated to young developing tissues. Microautoradiography showed that metaxylem parenchyma, secondary xylem parenchyma, and rays were the major areas of uptake from xylem vessels in the stem. Accumulation in phloem (high 14C concentrations in sieve tubes) took place in internodes subtending recently mature leaves. Little 14C from xylem-borne glutamine was found in phloem of mature leaves, which indicates restricted retransport of glutamine that did enter the leaf. In the primary tissues of the upper stem, most 14C was found in the phloem. Cottonwood stems have an efficient uptake and transfer system that enhances glutamine movement to developing tissues of the upper stem.