Generation of induced pluripotent stem cell lines from helper and cytotoxic T cells of healthy individuals

T lymphocytes are the most abundant mononuclear blood cells and can serve as a source for generating induced pluripotent stem cells (iPSCs) for disease modeling or drug development. Here, we report the derivation of two iPSC lines from CD4+ helper T cells and CD8+ cytolytic T cells, respectively. Th...

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Bibliographic Details
Published inStem cell research Vol. 69; p. 103113
Main Authors Kamaldinov, Timothy, Zhang, Linyi, Wang, Lin, Ye, Zhaohui
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.06.2023
Elsevier
Subjects
Cell Differentiation
Cell Line
Cellular Reprogramming
Embryonic Stem Cells - metabolism
Humans
Induced Pluripotent Stem Cells - metabolism
SOXB1 Transcription Factors - metabolism
T-Lymphocytes, Cytotoxic - metabolism
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Summary:T lymphocytes are the most abundant mononuclear blood cells and can serve as a source for generating induced pluripotent stem cells (iPSCs) for disease modeling or drug development. Here, we report the derivation of two iPSC lines from CD4+ helper T cells and CD8+ cytolytic T cells, respectively. The reprogramming was performed using Sendai virus encoding Klf-4, c-Myc, Oct-4 and Sox-2. Both iPSC lines displayed typical embryonic stem cell-like morphology and normal karyotype. Pluripotency was confirmed using immunocytochemistry methods and teratoma formation assay.
Bibliography:ObjectType-Article-1
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ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2023.103113