Generation of an integration-free induced pluripotent stem cell line, FJMUUHi002-A, from a Rett syndrome patient with a heterozygous mutation p. R133C in MeCP2

The human iPS cell line, hiPS-RTT (FJMUi002-A), is derived from peripheral blood mononuclear cells (PBMCs) from a 12-year-old female RTT patient carrying a heterozygous p. R133C (c.397C > T) mutation in the MeCP2 gene. The hiPS-RTT cell line was generated by non-integrative reprogramming vectors...

Full description

Saved in:
Bibliographic Details
Published inStem cell research Vol. 74; p. 103268
Main Authors Guo, Yu, Li, Jiaqi, Xie, Wenyi, Huang, Huaping, Li, Jin-Jing, Lin, Wanhui, Lu, Ying-Qian
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.02.2024
Elsevier
Subjects
Cell Differentiation
Cell Line
Child
Female
Humans
Induced Pluripotent Stem Cells - metabolism
Leukocytes, Mononuclear
Mutation - genetics
Rett Syndrome - genetics
Online AccessGet full text

Cover

More Information
Summary:The human iPS cell line, hiPS-RTT (FJMUi002-A), is derived from peripheral blood mononuclear cells (PBMCs) from a 12-year-old female RTT patient carrying a heterozygous p. R133C (c.397C > T) mutation in the MeCP2 gene. The hiPS-RTT cell line was generated by non-integrative reprogramming vectors encoding OCT3/4, SOX2, KLF4, and c-MYC and was free of genomically integrated reprogramming genes. The hiPS-RTT cell line had a normal karyotype, expressed pluripotency markers, and had capacity to form three germ layers in vitro and in vivo, which offering a useful resource to study the pathogenesis and treatment strategies of RTT.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2023.103268