Aerosol Vaccination with a Sendai Virus Temperature-Sensitive Mutant (HVJ-pB) Derived from Persistently Infected Cells

• Published in: The Journal of infectious diseases Vol. 162; no. 2; pp. 402 - 407
• Main Authors: Iwata, Hitoshi, Thgaya, Mitsuru, Matsumoto, Kazuo, Miyadai, Toshiaki, Yokochi, Thkashi, Kimura, Yoshinobu
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.08.1990; University of Chicago Press
• Subjects: Aerosols; Animals; Antibodies, Viral - biosynthesis; Biological and medical sciences; Cell lines; Fundamental and applied biological sciences. Psychology; Immunity, Cellular; Immunization, Passive; Infections; Inoculation; Interferons - biosynthesis; Lungs; Major Articles; Male; Mice; Mice, Inbred BALB C; Mice, Inbred CBA; Mice, Inbred DBA; Mice, Nude; Microbiology; Mutation; Parainfluenza Virus 1, Human - genetics; Parainfluenza Virus 1, Human - growth & development; Parainfluenza Virus 1, Human - immunology; Paramyxoviridae Infections - prevention & control; Respiratory System - microbiology; Sendai virus; Specific Pathogen-Free Organisms; Spleen cells; Turbinates; Vaccination; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Viral Vaccines - administration & dosage; Viral Vaccines - immunology; Virology; Viruses; Performance evaluation; Immunoprotection; Animal model; Sendai virus; Vaccination; Rodentia; Vaccine; Paramyxoviridae; Virus; Vertebrata; Experimental disease; Mammalia; Mouse; Aerosols; Paramyxovirus; Temperature sensitive mutation
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/162.2.402

Experimental infections of mice with a Sendai virus temperature-sensitive (ts) mutant (HVJ-pB) were studied. Infection with the ts mutant induced the priming effect of interferon production and both humoral and cellular immune responses, although the ts mutant virus neither multiplied satisfactorily in the respiratory tracts of mice nor caused appreciable histopathologic lesions. Inoculation with the ts mutant protected mice from subsequent challenge with a parental wild-type virus. The efficacy of this protection began as little as 1 day after vaccination and continued for at least 12 weeks. It is suggested that serum antibodies were efficacious in the nasal turbinates, while specific immune spleen cells act more protectively in the lungs.