Detection of Multiple Serovars of Chlamydia trachomatis in Genital Infections

• Published in: The Journal of infectious diseases Vol. 152; no. 5; pp. 985 - 989
• Main Authors: Barnes, Robert C., Suchland, Robert J., Wang, San-Pin, Kuo, Cho-Chou, Stamm, Walter E.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.11.1985; University of Chicago Press
• Subjects: Antibodies, Bacterial; Antibodies, Monoclonal; Bacterial diseases; Bacterial diseases of the genital system; Biological and medical sciences; Cell culture techniques; Cervix uteri; Cervix Uteri - microbiology; Chlamydia Infections - microbiology; Chlamydia trachomatis; Chlamydia trachomatis - classification; Chlamydia trachomatis - immunology; Chlamydia trachomatis - isolation & purification; Coinfection; Enzyme linked immunosorbent assay; Female; Genitalia; Homosexuality; Human bacterial diseases; Humans; Immunity; Infections; Infectious diseases; Jails; Male; Medical sciences; Monoclonal antibodies; Original Articles; Rectal Diseases - microbiology; Rectum - microbiology; Serotyping; Sexually Transmitted Diseases - microbiology; Uterine Cervical Diseases - microbiology; Infection; Human; Mixed infection; Chlamydiaceae; Chlamydia; Bacteriosis; Chlamydiales; Bacteria; Diagnostic; Chlamydiosis; Monoclonal antibody; Genital diseases
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/152.5.985

Eight genital isolates of Chlamydia trachomatis that each contained two different serovars were identified by typing with serovar-specific monoclonal antibodies. Mixed infections were confirmed by using serovar-specific monoclonal antibodies in an indirect immunofluorescence assay. For some isolates, fluorescein/rhodamine double-label indirect immunofluorescence with mixtures of IgM and IgG monoclonal antibodies to Chlamydia provided direct visual confirmation of mixed infection. Two isolates contained serovars D and F, and two E and F; one isolate contained serovars Ba and E, one D and J, one I′ and F, and one E and J. In a sample of 352 genital isolates of C trachomatis consecutively typed by dot ELISA with monoclonal antibodies, seven (20%) demonstrated mixed serovars.