Anti-pigmentation Effect of Serotonin Alkaloid Isolated from Korean Barnyard Millet (Echinochola utilis)

• Published in: Applied biological chemistry Vol. 55; no. 5; pp. 579 - 586
• Main Authors: Seo, W.D., National Institute of Crop Science, RDA, Miryang, Republic of Korea, Kim, J.Y., Sancheong Oriental Medicinal Herb Institute, Sancheong, Republic of Korea, Jang, K.C., National Institute of Crop Science, RDA, Miryang, Republic of Korea, Han, S.I., National Institute of Crop Science, RDA, Miryang, Republic of Korea, Ra, J.E., National Institute of Crop Science, RDA, Miryang, Republic of Korea, Oh, S.H., National Institute of Crop Science, RDA, Miryang, Republic of Korea, Lee, J.H., NAKDONG River Basin Environmental Office, Ministry of Environment, Changwon, Republic of Korea, Kim, Y.G., Sancheong Oriental Medicinal Herb Institute, Sancheong, Republic of Korea, Kang, H.J., National Institute of Crop Science, RDA, Miryang, Republic of Korea, Kim, B.J., National Institute of Crop Science, RDA, Miryang, Republic of Korea, Nam, M.H., National Institute of Crop Science, RDA, Miryang, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Applied Biological Chemistry 01.10.2012; Springer Nature B.V; 한국응용생명화학회
• Subjects: Applied Microbiology; Biological Techniques; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Cytotoxicity; ECHINOCHLOA FRUMENTACEA; Immunoblotting; Kojic acid; Melanin; Melanocytes; melanogenesis; Melanoma; Microphthalmia-associated transcription factor; Millet; Molecular docking; Mushrooms; n-p-coumaroyl serotonin; Original Article; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Phenolic compounds; Phenols; Pigmentation; Proteins; Serotonin; Toxicity; Tyrosinase; 농학; coumaroyl serotonin; melanogenesis; tyrosinase; barnyard millet; -
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2468-0842
• DOI: 10.1007/s13765-012-2112-7

Investigation of the melanogenesis inhibitory effect of barnyard millet grain extracts (BME) led to isolation of compounds against mushroom tyrosinase and B16 melanoma cells. Chromatography yielded five phenolic compounds, among which n-p-coumaroyl serotonin (1, CS) exhibited potent non-competitive inhibition (IC∧50 =8.8 μM) compared with that of kojic acid (IC∧50=14.6 μM) against mushroom tyrosinase. More importantly, CS decreased melanin content to eight times lower (IC∧50 =11.6 μM) in B16 melanocytes than that of feruloyl serotonin (2, FS, IC∧50 =86.5 μM) without cytotoxicity. CS significantly inhibited the protein expression of tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor using immunoblotting. Furthermore, molecular docking simulations were also carried out to investigate the structure related to the inhibitory activities. Therefore, BME can be used as a natural source of depigmentation to prevent melanogenesis.