Natural Deep Eutectic Solvent-Based Matrix Solid Phase Dispersion (MSPD) Extraction for Determination of Bioactive Compounds from Sandy Everlasting ( Helichrysum arenarium L.): A Case of Stability Study

• Published in: Plants (Basel) Vol. 11; no. 24; p. 3468
• Main Authors: Ivanović, Milena, Krajnc, Peter, Mlinarič, Aleš, Razboršek, Maša Islamčević
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.12.2022; MDPI
• Subjects: Acids; Adsorbents; Antioxidants; Bioactive compounds; Biological activity; Chloride; Chlorides; Chlorogenic acid; Choline; Dispersion; Elution; Environmental impact; extraction optimization; Flavonoids; Glucosides; Helichrysum arenarium; High performance liquid chromatography; Hydrogen bonds; Isomers; Lactic acid; Liquid chromatography; matrix solid phase dispersion extraction (MSPD); Methods; Naringenin; natural deep eutectic solvents (NADES); Optimization; Organic solvents; phenolic compounds; Phenols; Polyphenols; sandy everlasting; Solid phases; Solvents; Spectrophotometry; Stability; stability studies; Stationary phase; Ultrasonic imaging; Vortices; natural deep eutectic solvents (NADES); stability studies; extraction optimization; matrix solid phase dispersion extraction (MSPD); phenolic compounds; antioxidant activity; sandy everlasting
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants11243468

In the present study, vortex-assisted matrix solid-phase dispersion (VA-MSPD) extraction was used to isolate the major bioactive compounds from . To reduce the negative environmental impact of the conventionally used organic solvents, four different choline chloride-based natural deep eutectic solvents (NADES) were investigated as possible eluents. The most influential VA-MSPD extraction parameters: stationary phase (adsorbent), adsorbent/sample ratio, vortex time, and volume of extraction solvent were systematically optimized. Ultrasound-assisted extraction with 80% MeOH was used as the standard method for the comparison of results. The stability of the obtained extracts was studied over a period of 0 to 60 days at three different temperatures (-18 °C, 4 °C, and 25 °C). All extracts were evaluated both spectrophotometrically (determination of total phenolic content (TPC) and antioxidant activity by ABTS and FRAP assay) and chromatographically (HPLC-UV). NADES based on choline chloride and lactic acid (ChCl-LA) was selected as the most effective extractant, with a determined TPC value of its extract of 38.34 ± 0.09 mg GA/g DW (27% higher than the methanolic VA-MSPD extract) and high antioxidant activity. The content of individual phenolic compounds (chlorogenic acid, dicaffeoylquinic acid isomers, naringenin isomers, and chalcones) in the ChCl-LA extract, determined by HPLC-UV, was comparable to that of the conventionally obtained one. Moreover, the stabilization effect of ChCl-LA was confirmed for the studied compounds: chlorogenic acid, naringenin-4'-O-glucoside, tomoroside A, naringenin-5-O-glucoside, isosalipurposide, and naringenin. The optimum VA-MSPD conditions for the extraction of polyphenols were: florisil/sample ratio of 0.5/1, a vortex time of 2 min, and an elution volume of ChCl-LA of 10 mL.