Conditional Knockdown of Proteins Using Auxin-inducible Degron (AID) Fusions in Toxoplasma gondii

is a member of the deadly phylum of protozoan parasites called Apicomplexa. As a model apicomplexan, there is a great wealth of information regarding 's 8,000+ protein coding genes including sequence variation, expression, and relative contribution to parasite fitness. However, new tools are ne...

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Bibliographic Details
Published inBio-protocol Vol. 8; no. 4
Main Authors Brown, Kevin, Long, Shaojun, Sibley, L.
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 20.02.2018
Bio-protocol LLC
Subjects
Biology
Methods
Conditional knockdown
CRISPR
Degron
Auxin
Toxoplasma gondii
Parasite
Protein regulation
AID
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Summary:is a member of the deadly phylum of protozoan parasites called Apicomplexa. As a model apicomplexan, there is a great wealth of information regarding 's 8,000+ protein coding genes including sequence variation, expression, and relative contribution to parasite fitness. However, new tools are needed to functionally investigate hundreds of putative essential protein coding genes. Accordingly, we recently implemented the auxin-inducible degron (AID) system for studying essential proteins in . Here we provide a step-by-step protocol for examining protein function in using the AID system in a tissue culture setting.
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ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2728