Immunoelectron microscopic analysis of lysosomal deposits in α- N-acetylgalactosaminidase deficiency with angiokeratoma corporis diffusum

• Published in: Journal of dermatological science Vol. 29; no. 1; pp. 42 - 48
• Main Authors: Kanda, Akira, Tsuyama, Shinichiro, Murata, Fusayoshi, Kodama, Kazuo, Hirabayashi, Yoshio, Kanzaki, Tamotsu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.05.2002
• Subjects: alpha-N-Acetylgalactosaminidase; Animals; Antibodies, Monoclonal; Antigens, Tumor-Associated, Carbohydrate - analysis; Fabry Disease - enzymology; Female; Hexosaminidases - deficiency; Humans; Japan; Kanzaki disease; Lysosome; Lysosomes - ultrastructure; Male; Mice; Microscopy, Immunoelectron; Middle Aged; Tn antigen; α- N-Acetylgalactosaminidase; Japan; Kanzaki disease; α- N-Acetylgalactosaminidase; Tn antigen; Lysosome
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/S0923-1811(02)00005-1

α- N-Acetylgalactosaminidase (α-NAGA) deficiency with angiokeratoma corporis diffusum (AKCD) is one of the lysosomal storage diseases. GalNAcα1- O-Ser/Thr (Tn) is theoretically deposited in lysosomes, but substances with attached galactose and neuraminic (sialic) acid (T and sialosyl Tn, respectively) are excreted in patients’ urine. In this study, in two Japanese cases we analyzed the material accumulated in lysosomes using immunoelectron microscopy with mouse antibodies to Tn, sialosyl Tn and T (Thomsen–Friedenreich) antigens in order to find out what substance(s) is really deposited in lysosomes. We found that only GalNAcα1- O-Ser/Thr (Tn) was actually accumulated in vacuolated lysosomes of vascular endothelial cells, eccrine sweat gland cells, fibroblasts and pericytes. Galactosylation and sialylation of Tn appears to occur in cells other than those in the skin. The results suggest that this disease is caused by a single enzyme deficiency.