Capillary liquid chromatography of chlorophenoxy acid herbicides and their esters in apple juice samples after preconcentration on a cation exchanger based on polydivinylbenzene- N-vinylpyrrolidone

• Published in: Journal of Chromatography A Vol. 1076; no. 1; pp. 202 - 206
• Main Authors: Rosales-Conrado, N., León-González, M.E., Pérez-Arribas, L.V., Polo-Díez, L.M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 27.05.2005; Elsevier
• Subjects: Apple juice; Beverages - analysis; Biological and medical sciences; Capillary high performance liquid chromatography; Cation Exchange Resins; Cation exchanger polymer; Chlorophenoxy acid herbicides; food contamination; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; herbicide residues; herbicides; Herbicides - analysis; liquid chromatography; Malus; Pyrrolidinones - chemistry; Solid phase extraction; Styrenes - chemistry; Solid phase extraction; Cation exchanger polymer; Apple juice; Capillary high performance liquid chromatography; Chlorophenoxy acid herbicides; Trace analysis; Capillary column; Benzene(divinyl) copolymer; Chemical analysis; Pesticides; HPLC chromatography; Fruit juice; Herbicide; Chemical enrichment; Apple; Ultraviolet detector; Sample preparation; Pyrrolidone(vinyl) copolymer; Adsorbent; Cation exchanger; Quantitative analysis
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2005.04.026

A capillary liquid chromatography (cLC) method with gradient elution has been used to determine chlorophenoxy acid herbicides: 2,4-dichlorophenoxyacetic acid, 4-chloro-2-methylphenoxyacetic acid, 2-(2,4-dichlorophenoxy)propanoic acid, 2-(4-chloro-2-methylphenoxy)propanoic acid, 4-(2,4-dichlorophenoxy)butanoic acid, 4-(4-chloro-2-methylphenoxy)butanoic acid, 2-(2,4,5-trichlorophenoxy)propanoic acid, 2,4-dichlorophenoxyacetic-1-methyl ester and 2,4-dichlorophenoxyacetic-1-butyl ester in spiked apple juice samples with amounts between 0.025 and 0.150 mg kg −1 of each herbicide. Clean-up and preconcentration of acid and esters were carried out in an Oasis MCX polymer. Detection limits obtained by cLC, between 0.005 and 0.018 mg kg −1, allowed the determination of chlorophenoxy acids and their esters in apple juice samples around the levels permitted by the European Regulations, with recoveries in the range 84–99% and RSDs between 1 and 4%.