Determination of glutathione content in grape juice and wine by high-performance liquid chromatography with fluorescence detection

• Published in: Analytica chimica acta Vol. 674; no. 2; pp. 239 - 242
• Main Authors: Janeš, Lucija, Lisjak, Klemen, Vanzo, Andreja
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 03.08.2010; Elsevier
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Exact sciences and technology; Fluorescence; Glutathione; Glutathione - analysis; Grape juice; High-performance liquid chromatography with fluorescence detection; Limit of Detection; Other chromatographic methods; Pre-column derivatization; Vitaceae; Vitis - chemistry; Wine; Wine - analysis; Wine; High-performance liquid chromatography with fluorescence detection; Grape juice; Pre-column derivatization; Glutathione; Fluorescence detector; Uncertainty; Enzyme; Sample; Use; HPLC chromatography; Ice; Acetate; Method; Derivatization; High-performance liquid chromatography; Fruit juice; Sample preparation; with fluorescence detection; Sodium; Detection limit; Linearity; Oxidation; Methanol
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2010.06.040

A modified preparation of sample was developed for the determination of glutathione content in grape juice and wine by high-performance liquid chromatography with fluorescence detection, using on-line pre-column derivatization. Ice-cold deoxygenated methanol was used to deactivate the oxidation enzymes in juices or wines and keep the glutathione stable. The optimum recovery of glutathione content in grape juice and wine was obtained when either the sample of grape juice or wine was mixed in ice-cold deoxygenated methanol in the ratio 10:90 (v:v) and further diluted in sodium acetate buffer in the ratio 1:1 (v:v). The optimized method was validated for linearity, limit of detection, limit of quantification, precision and uncertainty. According to the validation data the method is appropriate for the determination of glutathione content in grape juice and wine. Glutathione contents in grape juices made from White Muscat grapes and Sauvignon Blanc wines were analysed. The average glutathione content in 28 young Sauvignon Blanc wines was 12.5 mg L −1.