Is direct method of low density lipoprotein cholesterol measurement appropriate for targeting lipid lowering therapy?

• Published in: International journal of cardiology Vol. 142; no. 1; pp. 105 - 107
• Main Authors: Can, Murat, Acikgoz, Serefden, Mungan, Gorkem, Ugurbas, Ebru, Ankarali, Handan, Sumbuloglu, Vildan, Demirtas, Selda, Karaca, Levent
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 25.06.2010; Elsevier
• Subjects: Aged; Biological and medical sciences; Cardiology. Vascular system; Cardiovascular; Cholesterol - blood; Cholesterol, LDL - blood; Direct homogeneous LDL cholesterol assay; Drug Delivery Systems - methods; Female; Friedewald formula; Hematologic Tests - methods; Humans; LDL-cholesterol; Male; Medical sciences; Middle Aged; Triglycerides - blood; Friedewald formula; LDL-cholesterol; Direct homogeneous LDL cholesterol assay; Measurement; Targeting; Cholesterol LDL; Lipids; Cardiovascular disease; Assay; Lipoprotein LDL; Direct method; Treatment; Cardiology
• ISSN: 0167-5273; 1874-1754
• DOI: 10.1016/j.ijcard.2008.11.141

Abstract Objectives The aim of this study was to compare the Friedewald Formula with direct homogeneous low density lipoprotein cholesterol (LDL-C) assay for the detection of LDL-C levels. Methods Fasting serum samples were obtained for lipid analysis from 1001 patients. Total cholesterol (TC) and triglyceride (TG) levels were measured with enzymatic methods and the measurements of high density lipoprotein cholesterol (HDL-C) and LDL-C levels were detected using direct methods. Results The mean levels of serum TC, TG, HDL-C and LDL-C were detected with in the reference range. The LDL-C estimated by the Friedewald formula was significantly correlated ( P < 0.01) with the direct method but there was a negative bias among them. Conclusion Laboratories cannot use direct method as a substitute for Friedewald formula because direct method has not been standardized in large populations and increase cholesterol assay costs.