Characterization of the human induced pluripotent stem cell (iPSC) SZGJMSi004-A line from a 28-year-old Han male patient with depression

Peripheral blood mononuclear cells (PBMCs) from a 28-year-old male patient with unipolar depression were reprogrammed with reprogramming factors by electroporation. The pluripotency of transgene-free induced pluripotent stem cells (iPSCs) was verified by immunofluorescence staining for pluripotency...

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Published inStem cell research Vol. 77; p. 103428
Main Authors Yin, Xuyuan, Cai, Yuan, Jia, Ningning, Hui, Li, Zhu, Zhenhua
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.06.2024
Elsevier
Subjects
Adult
Cell Differentiation
Cell Line
Cellular Reprogramming
Depression
Han population
Humans
Induced pluripotent stem cells
Induced Pluripotent Stem Cells - metabolism
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - metabolism
Male
Peripheral blood mononuclear cells
Peripheral blood mononuclear cells
Depression
Han population
Induced pluripotent stem cells
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Summary:Peripheral blood mononuclear cells (PBMCs) from a 28-year-old male patient with unipolar depression were reprogrammed with reprogramming factors by electroporation. The pluripotency of transgene-free induced pluripotent stem cells (iPSCs) was verified by immunofluorescence staining for pluripotency markers, and these iPSCs were able to differentiate into the 3 germ layers in vitro. These iPSCs also showed normal karyotypes. Thus, we believe that these iPSCs could be valuable models for exploring the underlying biological mechanism of depression and the safety of antidepressants through the use of iPSCs differentiated into different kinds of neurons or brain organoids.
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ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2024.103428