Distance determination from dysprosium induced relaxation enhancement: a case study on membrane-inserted WALP23 polypeptides

Membrane incorporated synthetic α-helical polypeptides labelled with Dy(III) chelate complexes and nitroxide radicals were studied by the inversion recovery (IR) technique and Dy(III)-nitroxide distances were obtained. A comparison of obtained distances with the previously reported Gd(III)-nitroxide...

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Published inMolecular physics Vol. 111; no. 18-19; pp. 2824 - 2833
Main Authors Lueders, Petra, Razzaghi, Sahand, Jäger, Heidrun, Tschaggelar, René, Hemminga, Marcus A., Yulikov, Maxim, Jeschke, Gunnar
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 01.10.2013
Taylor & Francis Ltd
Subjects
biomacromolecules
Calibration
dipolar spectroscopy
distance measurements
EPR/ESR
inversion recovery
lanthanide ions
Polypeptides
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Summary:Membrane incorporated synthetic α-helical polypeptides labelled with Dy(III) chelate complexes and nitroxide radicals were studied by the inversion recovery (IR) technique and Dy(III)-nitroxide distances were obtained. A comparison of obtained distances with the previously reported Gd(III)-nitroxide double electron-electron resonance (DEER) calibration data was performed and revealed reliability of the IR-based technique for the distance determination in membrane-incorporated biomacromolecules. The presented distance determination technique is 'spectroscopically orthogonal' to DEER-based distance measurements and can be potentially combined with DEER to study multiply spin-labelled biomacromolecules. The key steps of the data processing, the types of obtained distance information and the areas of possible application of the technique are discussed.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0026-8976
1362-3028
DOI:10.1080/00268976.2013.806683