Allozyme and microsatellite diversity in natural and domestic populations of turbot (Scophthalmus maximus) in comparison with other Pleuronectiformes

• Published in: Canadian journal of fisheries and aquatic sciences Vol. 59; no. 9; pp. 1460 - 1473
• Main Authors: Bouza, C, Presa, P, Castro, J, Sánchez, L, Martínez, P
• Format: Journal Article
• Language: English
• Published: Ottawa, Canada NRC Research Press 01.09.2002; National Research Council of Canada
• Subjects: Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Exploitation and management of natural biological resources (hunting, fishing and exploited populations survey, etc.); Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Marine; Platichthys flesus; Pleuronectiformes; Population genetics, reproduction patterns; Scophthalmus maximus; Scophthalmus rhombus; Vertebrata; Europe; Northeast Atlantic; Spain; ANE, Spain, Galicia; ANE, Spain, Cantabria; Natural population; Scophthalmus maximus; Genetic variability; Genetic marker; Interspecific comparison; Domestication; Platichthys flesus; Genetic diversity; Allozyme; Biodiversity; Population genetics; Coastal zone; Marine environment; Vertebrata; Fishery; Pisces; Microsatellite DNA
• ISSN: 0706-652X; 1205-7533
• DOI: 10.1139/f02-114

Twelve microsatellite and 28 allozyme loci were employed to analyse genetic diversity in natural and domestic populations of turbot (Scophthalmus maximus) from northwest Spain in comparison with other flatfish species with similar habitat, life history, and geographic distribution—the brill (Scophthalmus rhombus) and the flounder (Platichthys flesus). These species had shown much higher allozyme diversity than turbot in previous studies, and were used as a reference to check for putative historical bottlenecks in turbot. Significantly lower genetic variability in turbot than in brill and flounder was confirmed with allozymes, but not with the highly variable microsatellite loci. This intermarker discrepancy could be explained by different mutation rates in relation with historical bottlenecks along turbot evolution. A significantly lower genetic diversity was observed in a domestic strain of turbot than in natural populations of this species. This sample evidenced a strong family structure from microsatellite data, which suggests caution against the use of commercial batches for broodstock foundation in turbot farming. A strong concordance was found across the two categories of markers used when analysing the pattern of genetic subdivision at a local scale within the three species analysed, low and nonsignificant genetic differentiation being observed between Atlantic and Cantabric areas.