Radio frequency induced hyperthermia mediated by dextran stabilized LSMO nanoparticles: in vitro evaluation of heat shock protein response

• Published in: Nanotechnology Vol. 24; no. 1; p. 015102
• Main Authors: Bhayani, K R, Rajwade, J M, Paknikar, K M
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 11.01.2013; Institute of Physics
• Subjects: Biological and medical sciences; Biotechnology; Cancer; Cell Death - drug effects; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Shape - drug effects; Cross-disciplinary physics: materials science; rheology; Dextrans; Dextrans - chemistry; Electrophoresis, Polyacrylamide Gel; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Heat shock proteins; Heat-Shock Proteins - metabolism; Heat-Shock Response - drug effects; Heating; HSP70 Heat-Shock Proteins - metabolism; HSP90 Heat-Shock Proteins - metabolism; Humans; Hyperthermia; Hyperthermia, Induced; Lanthanum - toxicity; Manganese Compounds; Markers; Materials science; Metal Nanoparticles - toxicity; Methods. Procedures. Technologies; Microscopy, Phase-Contrast; Nanocrystalline materials; Nanoscale materials and structures: fabrication and characterization; Others; Oxides - toxicity; Physics; Radio frequencies; Radio Waves; Strontium - toxicity; Time Factors; Various methods and equipments; Dextran; Nanoparticles; Proteins; Macromolecules; Morphology; Hyperthermia; Nanostructured materials; Heat treatments; Comparative study
• ISSN: 0957-4484; 1361-6528
• DOI: 10.1088/0957-4484/24/1/015102

Dextran stabilized La0.7Sr0.3MnO3 (Dex-LSMO) is an alternative cancer hyperthermia agent holding considerable promise. Here, we have carried out a comparative study on radio frequency (∼264 kHz) induced Dex-LSMO mediated heating and extraneous heating (mimicking generalized hyperthermia) in terms of changes in the morphology, proliferation pattern and induction of heat shock proteins in a human melanoma cell line (A375). Our results clearly show that the cellular effects seen with extraneous heating (60 min at 43 °C) could be reproduced by just six minutes of radio frequency induced Dex-LSMO mediated heating. More importantly, the observed enhanced levels of HSP 70 and 90 (molecular markers of heat shock that trigger favorable immunological reactions) seen with Dex-LSMO mediated heating were comparable to extraneous heating. These results suggest the possible utility of Dex-LSMO as a cancer hyperthermia agent.