IgG Subclass Antibodies to Herpes Simplex Virus

• Published in: The Journal of infectious diseases Vol. 151; no. 5; pp. 929 - 936
• Main Authors: Coleman, R. Marie, Nahmias, André J., Williams, Susan C., Phillips, Donald J., Black, Charlotte M., Reimer, Charles B.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.05.1985; University of Chicago Press
• Subjects: Adult; Animals; Antibodies; Antibodies, Monoclonal; Antibodies, Viral - classification; Antibody Specificity; Antigen-Antibody Reactions; Antigens; Biological and medical sciences; Blood Proteins - immunology; Enzyme linked immunosorbent assay; Genitalia; Herpes Simplex - immunology; herpes simplex virus; Human herpesvirus 1; Human herpesvirus 2; Human viral diseases; Humans; Immunoglobulin Allotypes - immunology; Immunoglobulin G - classification; Immunoglobulin G - immunology; Infant, Newborn; Infections; Infectious diseases; Medical sciences; Mice; Molecules; Monoclonal antibodies; Myeloma Proteins - immunology; Original Articles; Simplexvirus; Simplexvirus - immunology; Viral diseases; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye; Infection; Virus; Immunopathology; Skin disease; Α-Herpesvirinae; Antibody; Herpesviridae; Viral disease; Herpes; IgG; Subgroup; Herpesvirus hominis
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/151.5.929

Several mouse monoclonal antibodies specific for human IgGl, IgG2, IgG3, and IgG4 were evaluated by enzyme-linked immunosorbent assay to detect human IgG subclass antibodies to herpes simplex virus (HSV) antigens. The variable results with different monoclonal antibodies point to the need for well-characterized reagents in the study of antibody responses to infectious agents. The 204 sera tested were obtained from 157 patients with various forms of clinically manifest HSV infections and from several controls. IgGI antibodies were demonstrated in almost all HSV-infected subjects and were the first antibodies to appear in primary genital infections. IgG2, IgG3, and IgG4 antibodies were detected in acute-phase sera, most often in patients with recurrent genital herpes but in none of those with primary infections. IgG4 antibodies occurred significantly more frequently in sera from men than in those from women with recurrent genital infections.