Label-free and real-time detection of specific binding of IgG proteins by oblique-incidence reflectivity difference method

The entire interaction process between anti-mouse immunoglobulin-G （IgG） and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference （OIRD） method. We achieved the two-dimensional scanning images of OIRD Re{△p-△s} intens...

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Published inChinese science bulletin Vol. 57; no. 22; pp. 2898 - 2900
Main Authors Sun, Yue, He, LiPing, Dai, Jun, Wang, JingYi, Lü, HuiBin, Wang, ShuFang, Jin, KuiJuan, Zhou, YueLiang, Yang, GuoZhen
Format Journal Article
LanguageEnglish
Published Heidelberg Springer-Verlag 01.08.2012
SP Science China Press
Subjects
Binding
Chemistry/Food Science
Earth Sciences
Engineering
G蛋白
Humanities and Social Sciences
immunoglobulin G
Life Sciences
mice
microarray technology
Monitors
multidisciplinary
Physics
Proteins
Real time
Reflectivity
Scanning
Science
Science (multidisciplinary)
Two dimensional
免疫球蛋白G
反射率
实时检测
斜入射
无标记
特异性结合
生物分子相互作用
biomolecular interactions
oblique-incidence reflectivity difference (OIRD)
label-free and real-time detection
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Summary:The entire interaction process between anti-mouse immunoglobulin-G （IgG） and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference （OIRD） method. We achieved the two-dimensional scanning images of OIRD Re{△p-△s} intensities before and after interactions as well as the curves of reaction dynamic processes. The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays, but also can real-time monitor the kinetic processes of biomolecular interactions,
Bibliography:oblique-incidence reflectivity difference （OIRD）; label-free and real-time detection; biomolecular interactions
The entire interaction process between anti-mouse immunoglobulin-G （IgG） and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference （OIRD） method. We achieved the two-dimensional scanning images of OIRD Re{△p-△s} intensities before and after interactions as well as the curves of reaction dynamic processes. The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays, but also can real-time monitor the kinetic processes of biomolecular interactions
11-1785/N
SUN Yue, HE LiPing, DAI Jun, WANG JingYi, Lǖ HuiBin , WANG ShuFang , JIN KuiJuan, ZHOU YueLiang, YANG GuoZhen（ 1. College of Physics Science and Technology, Hebei University, Baoding 071002, China; 2. Beijing National Laboratory,for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China）
http://dx.doi.org/10.1007/s11434-012-5263-3
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1001-6538
1861-9541
DOI:10.1007/s11434-012-5263-3