Engineering of a novel D–A type fluorophore with hydrogen bond-induced enhanced emission property for sensitively detecting endogenous HOCl in living cells and tissues

Fluorescence imaging has been widely employed for biomedical research and clinical diagnostics. With ease of synthesis and excellent photophysical properties, D–A type fluorophores are widely designed for fluorescence imaging. However, traditional D–A type fluorophores are solvatochromic which reduc...

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Published inAnalytical and bioanalytical chemistry Vol. 415; no. 18; pp. 4185 - 4196
Main Authors Wang, Si-Min, Lou, Xiaofeng, Xu, Shuai, Song, Zhi-Ling, Ren, Tian-Bing, Huan, Shuang-Yan, Yuan, Lin, Zhang, Xiao-Bing
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2023
Springer
Springer Nature B.V
Subjects
Analysis
Analytical Chemistry
Biochemistry
Brightness
Characterization and Evaluation of Materials
Chemical compounds
Chemical synthesis
Chemistry
Chemistry and Materials Science
Chlorides
Cytotoxicity
Design
Dichloropropane
Dyes
Electron donor-acceptor complexes
Engineering
Experiments
Fluorescence
Fluorescent indicators
Fluorophores
Food Science
Hydrogen
Hydrogen bonds
Identification and classification
Imaging
Laboratory animals
Laboratory Medicine
Medical research
Methods
Monitoring/Environmental Analysis
Paper in Forefront
Properties
Young Investigators in (Bio-)Analytical Chemistry 2023
China
HOCl detecting
Hydrogen bond-induced enhanced emission
D–A fluorophore
Subcellular imaging
Fluorescent probe
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Summary:Fluorescence imaging has been widely employed for biomedical research and clinical diagnostics. With ease of synthesis and excellent photophysical properties, D–A type fluorophores are widely designed for fluorescence imaging. However, traditional D–A type fluorophores are solvatochromic which reduces the fluorescence brightness in the biological system. To solve this problem and build on our previous work, we devised a novel HIEE fluorophore MTC with typical anti-solvatochromic fluorescence. Furthermore, the activated fluorescent probe designed based on MTC showed excellent imaging performance. We believe that the strategy based on the fluorophores with typical anti-solvatohromic fluorescence can be a useful platform for designing fluorescent probes for high-brightness imaging in the biological system. Graphical Abstract
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ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-023-04550-9