Engineering of a novel D–A type fluorophore with hydrogen bond-induced enhanced emission property for sensitively detecting endogenous HOCl in living cells and tissues
Fluorescence imaging has been widely employed for biomedical research and clinical diagnostics. With ease of synthesis and excellent photophysical properties, D–A type fluorophores are widely designed for fluorescence imaging. However, traditional D–A type fluorophores are solvatochromic which reduc...
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Published in | Analytical and bioanalytical chemistry Vol. 415; no. 18; pp. 4185 - 4196 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.07.2023
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Fluorescence imaging has been widely employed for biomedical research and clinical diagnostics. With ease of synthesis and excellent photophysical properties, D–A type fluorophores are widely designed for fluorescence imaging. However, traditional D–A type fluorophores are solvatochromic which reduces the fluorescence brightness in the biological system. To solve this problem and build on our previous work, we devised a novel HIEE fluorophore MTC with typical anti-solvatochromic fluorescence. Furthermore, the activated fluorescent probe designed based on MTC showed excellent imaging performance. We believe that the strategy based on the fluorophores with typical anti-solvatohromic fluorescence can be a useful platform for designing fluorescent probes for high-brightness imaging in the biological system.
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1618-2642 1618-2650 |
DOI: | 10.1007/s00216-023-04550-9 |